Name: APAgene™ GOLD-RT Genome Walking Kits
Price: Inquiry

- Overview
  - The APAgene™ GOLD Genome Walking Kit can enable rapid isolation of unknown sequences which flank known gene sequences. Examples include:
    •Genomic sequences flanking transgenes (including T-DNAs, gene traps, transposons), sequence-tagged sites (STSs) or expressed sequence tags (ESTs);
    •Insert ends of large clones such as P1, YAC and BAC DNAs;
    •5’ promoter control regions and 3’ transcriptional terminators of cDNAs;
    •cDNA walking, 5’ and 3’ RACE from first-strand cDNAs;
    •Other localized sequences flanking known sequences from large clones and genomic DNAs. PCR products can directly be used for sequencing, cloning or as probes.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Components
    • APAgeneTM 2X Premix
    • 5X DRT binding mixture
    • DRT Primer A
    • DRT Primer B
    • DRT Primer C
    • DRT Primer D
    • UAP-N1 (20 pmol/μL)
    • UAP-N2 (20 pmol/uL)
    
    * For research use only
- Applications
  - Application Description
    
    APAgene™ GOLD Genome Walking Kits are designed to rapidly (1 day!) and reliably amplify unknown genomic DNA using our patented APA technology. APAgene™ GOLD is the ideal genomic tool because it allows you to carry out an entire range of molecular genetic experiments:
    Isolate unknown genomic sequences flanking transgenes (including T-DNAs, gene traps, transposons), sequence-tagged sites (STSs) or expressed sequence tags (ESTs).
    Isolate localized sequences flanking known sequences from large clones and genomic DNAs.
    Sequence insert ends of large clones such as P1, YAC and BAC DNAs.
    Isolate unknown 5' promoter control regions and 3' transcriptional terminators of cDNAs.
    Isolate unknown 5' and 3' RACE from first-strand cDNAs.
- Reference
  - A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening, Kenneth Manning et al., Nature Genetics, Volume: 38, Issue: 8, Date: 2006 07 28, Pages: 948-522
    A Na+: H+ Antiporter and a Molybdate Transporter Are Essential for Arsenite Oxidation in Agrobacterium tumefaciens, Journal of Bacteriology, February 2006, p. 1577-1584, Vol. 188, No. 4, Des R. Kashyap et al.
    Y chromosome microsatellite isolation from BAC clones in the greater white-toothed shrew (Crocidura russula), Lawson Handley, L.J., Perrin, N., 2006, Molecular Ecology Notes 60, 276-279.
    Complex Regulation of Arsenite Oxidation in Agrobacterium tumefaciens, Journal of Bacteriology, February 2006, p. 1081-1088, Vol. 188, No. 3, Des R. Kashyap et al.
    Identification and regulatory analysis of rainbow trout tapasin and tapasin-related genes, Immunogenetics 2006 Feb; 58(1) :56-69. Landis ED, Palti Y, Dekoning J, et al.
    Detection and Characterization of Amplified Fragment Length Polymorphism Markers for Clinical Mastitis in Canadian Holsteins, J Dairy Sci. 2006 Sep;89 (9):3653-63., B. S. Sharma et al.
    Homologs of CD83 from Elasmobranch and Teleost Fish, Yuko Ohta et al., The Journal of Immunology, 2004, 173: 4553-4560.

APAgene™ GOLD-RT Genome Walking Kits

APAgene™ GOLD-RT Genome Walking Kits

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