Name: Anti-Vimentin antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Fizzy-Related Protein Homolog; Fzr; Cdc20-Like Protein 1; Cdh1/Hct1 Homolog; Hcdh1 (Gene ID:51343; UniProt:Q9UM11)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; Flow Cytometry; IF; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:4000.
    WB-Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates. Recommended concentration: 0.1-2µg/ml.
    IHC-Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
    IF-FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended concentration: 5µg/ml.