Name: Anti-UBE2C/UBCH10 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Ubiquitin-Conjugating Enzyme E2 C; (E3-Independent E2 Ubiquitin-Conjugating Enzyme C; E2 Ubiquitin-Conjugating Enzyme C; Ubch10; Ubiquitin Carrier Protein C; Ubiquitin-Protein Ligase C (Gene ID:11065; UniProt:O00762)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry; IP
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx.19KDa band observed in lysates of cell lines HeLa and HEK293. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
    IF-Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.