Name: Anti-TNF-Alpha antibody
Price: 224.00 USD

Overview
- TNF-alpha is a protein encoded by the TNF gene which is approximately 25, 6 kDa. TNF-alpha is localised to the cell membrane. It is involved in PEDF induced signalling, TNFR1 pathway, allograft rejection and apoptosis modulation and signalling. It is a cytokine that is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, lipid metabolism, and coagulation. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF-alpha is expressed in the blood, lung, spleen, liver and heart. Mutations in the TNF gene may result in psoriatic arthritis. STJ96054 was affinity purified. This polyclonal antibody binds to endogenous TNF-alpha.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human TNF-alpha
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Tumor Necrosis Factor; Cachectin; Tnf-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; Tnf-A [Cleaved Into-Tumor Necrosis Factor-Membrane Form; N-Terminal Fragment; Ntf-Intracellular Domain 1; Icd1-Intracellular Domain 2; Icd2-C-Domain 1-C-Domain 2-Tumor Necrosis Factor-Soluble Form] (Gene ID:7124; UniProt:P01375)
  
  * For research use only

Applications

Application

WB; IHC (paraffin); IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:10000

We Also Recommend

Anti-TN alpha-IP 2 antibody

$224

Anti-TNF-Beta antibody

$224

Anti-TNNI3K antibody

$297

Anti-TNNI3K antibody

$224

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using TNFA Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunofluorescence analysis of HepG2 cells, using TNFA Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-TNF-Alpha antibody

Anti-TNF-Alpha antibody