Name: Anti-TIMP-1 antibody
Price: 224.00 USD

Overview
- TIMP-1 is a protein encoded by the TIMP-1 gene which is approximately 23, 2 kDa. TIMP-1 is involved in the GPCR pathway, phospholipase-C pathway, apoptotic pathways in synovial fibroblasts, ERK signalling and the CREB pathway. It functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signalling cascades via CD63 and ITGB1, it also acts as an inhibitor of MMPs which are groups of peptides involved in extracellular matrix degradation. TIMP-1 is secreted and is only active in the presence of disulphide bonds. It is ubiquitously expressed in numerous cells and tissues of the body. Mutations in the TIMP-1 gene may result in chronic venous leg ulcers and oral submucous fibrosis. STJ96023 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of TIMP-1 protein.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human TIMP-1
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Metalloproteinase Inhibitor 1; Erythroid-Potentiating Activity; Epa; Fibroblast Collagenase Inhibitor; Collagenase Inhibitor; Tissue Inhibitor Of Metalloproteinases 1; Timp-1 (Gene ID:7076; UniProt:P01033)
  
  * For research use only

Applications

Application

IF; WB; IHC (paraffin); ELISA

Application	Dilutions
IF	1:50-200
WB	1:500-1:2000
ELISA	1:10000

We Also Recommend

Anti-TIMP-2 antibody

$1,012

Anti-TIMP-2 antibody

$224

Anti-TIMP-3 antibody

$224

Anti-TIMP-4 antibody

$224

Anti-TIMP-4 antibody

$224

Immunohistochemical analysis of paraffin-embedded Human uterus. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human uterus. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Western blot analysis of lysates from rat brain cells, using TIMP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of human-uterus tissue. 1, TIMP-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-TIMP-1 antibody

Anti-TIMP-1 antibody