Name: Anti-TGF Beta antibody
Price: 224.00 USD

Overview
- TGF-beta 1 is a protein encoded by the TGFB1 gene which is approximately 44, 3 kDa. TGF-beta 1 is secreted into the extracellular space and matrix and is involved in apoptotic pathways in synovial fibroblasts, SMAD2/3 MH2 domain mutants in cancer, Th17 differentiation pathway, GPCR pathway and ERK signalling. This protein falls under the TGF-beta superfamily which bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. It is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. TGF-beta 1 is highly expressed in bone. Mutations in the TGFB1 gene may result in Camurati-Engelmann disease and Cystic Fibrosis, Renal Fibrosis. STJ95997 was developed from clone 5D2. It was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This mouse monoclonal antibody detects endogenous levels of TGF-beta 1.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human TGFbeta1
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Transforming Growth Factor Beta-1 Proprotein [Cleaved Into-Latency-Associated Peptide; Lap-Transforming Growth Factor Beta-1; Tgf-Beta-1] (Gene ID:7040; UniProt:P01137)
  
  * For research use only

Applications

Application

WB; IHC (paraffin); IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:20000

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Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TGF beta1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-lung tissue. 1, TGF Beta 1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-TGF Beta antibody

Anti-TGF Beta antibody