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Overview
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Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), expressed in Streptomyces avidinii, that exhibits an intrinsic affinity for streptavidin and biotin and is used for the detection and purification of recombinant proteins. It offers a versatile tool both for the rapid isolation of a functional gene product and for its detection or molecular interaction due to it being short, biologically inert, proteolytically stable and not interfering with membrane translocation or protein folding. STJ97057 was developed from clone Mix and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects Strep Tag II recombinant protein.
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