Name: Anti-SPA1/SIPA1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Signal-Induced Proliferation-Associated Protein 1; Sipa-1; Gtpase-Activating Protein Spa-1; P130 Spa-1 (Gene ID:6494; UniProt:Q96FS4)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid. The observed molecular weight corresponds to earlier findings in literature with different antibodies :28081-8; PMID: 9346962). Recommended concentration: 1-3µg/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.

STJ70500 staining (1µg/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-SPA1/SIPA1 antibody

Anti-SPA1/SIPA1 antibody

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