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Overview
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Sox-9 is a protein encoded by the SOX9 gene which is approximately 56, 2 kDa. Sox-9 is localised to the nucleus. It is involved in signalling by wnt, cAMP signalling pathway and embryonic and induced pluripotent stem cell differentiation pathways and lineage-specific markers. It plays an important role in the normal skeletal development and may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Sox-9 is expressed in the nervous system, eye, bone, intestine and pancreas. Mutations in the SOX9 gene may result in campomelic dysplasia. STJ95736 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Sox-9 protein.
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Overview
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SOX9 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, treated with PBS 60', using SOX9 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of various cells using Sox-9 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunofluorescence analysis of A549 cells, using SOX9 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of rat-spleen tissue. 1, Sox-9 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-spleen tissue. 1, Sox-9 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-lung tissue. 1, Sox-9 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-lung tissue. 1, Sox-9 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of Hela cell. 1, Sox-9 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-Sox-9 antibody
Anti-Sox-9 antibody
Catalog Number:
A001051106STJ
Mfr. No.:
STJ95736
Price:
$224
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