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Overview
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Smad4 is a protein encoded by the SMAD4 gene which is approximately 60, 4 kDa. Smad4 is localised to the cytoplasm and nucleus. It is involved in transcriptional activity of SMAD2/SMAD3-SMAD4 heterotrimer, the TGF-beta pathway, signalling by BMP and the cell cycle. It is phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signalling. It forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. Smad4 is expressed in the cells of the nervous system, liver, muscle, intestine and pancreas. Mutations in the SMAD4 gene may result in pancreatic cancer and juvenile Polyposis syndrome. STJ95702 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Smad4 protein.
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Overview
Western blot analysis of the lysates from HT-29 cells using Smad4 antibody.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, Smad4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from HeLa, COS7, HepG2, and HUVEC cells, using Smad4 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Smad4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, Smad4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of mouse-kidney tissue. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of mouse-kidney tissue. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of mouse-lung tissue. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of mouse-lung tissue. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-lung tissue. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-lung tissue. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of Hela cell. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-Smad4 antibody
Anti-Smad4 antibody
Catalog Number:
A001051072STJ
Mfr. No.:
STJ95702
Price:
$224
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