Application Description
ELISA-antibody detection limit dilution 1:4000.
WB-Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC-In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3µg/ml.