Name: Anti-Rb antibody
Price: 224.00 USD

Overview
- Rb is a protein encoded by the RB1 gene which is approximately 106, 1 kDa. Rb is localised to the nucleus and is involved in CDK-mediated phosphorylation and removal of Cdc6 and mitotic cell cycle. It stabilizes constitutive heterochromatin to maintain the overall chromatin structure and is a key regulator of entry into cell division that acts as a tumor suppressor. It also promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Rb is expressed in the retina. Mutations in the RB1 gene may result in retinoblastoma and bladder cancer. STJ95388 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Rb protein.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human Rb around the non-phosphorylation site of S780.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Retinoblastoma-Associated Protein; P105-Rb; P110-Rb1; Prb; Rb; Pp110 (Gene ID:5925; UniProt:P06400)
  
  * For research use only

Applications

Application

WB; IHC (paraffin); IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:20000

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Western blot analysis of various cells using Rb Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Retinoblastoma Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Western blot analysis of lysates from HepG2, Jurkat, and HUVEC cells, using Retinoblastoma Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Rb antibody

Anti-Rb antibody

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Western blot analysis of various cells using Rb Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Retinoblastoma Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Western blot analysis of lysates from HepG2, Jurkat, and HUVEC cells, using Retinoblastoma Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Rb antibody

Anti-Rb antibody

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