Name: Anti-PU.1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; rat
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Transcription Factor Pu.1; 31 Kda-Transforming Protein (Gene ID:6688; UniProt:P17947)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx 40kDa band observed in lysates of DAUDI and K562. The observed molecular weight corresponds to earlier findings in literature with different antibodies :19552-9.; PMID: 10867017). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
    IHC-Paraffin embedded Human Spleen and Colon. Recommended concentration: 3.75µg/ml.