Name: Anti-Proenkephalin antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    mouse; rat
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Proenkephalin-A Cleaved Into-Synenkephalin-Met-Enkephalin; Opioid Growth Factor; Ogf-Penk(114-133-Penk(143-183-Met-Enkephalin-Arg-Gly-Leu-Leu-Enkephalin-Penk(237-258-Met-Enkephalin-Arg-Phe (Gene ID:5179; UniProt:P01210)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.
    IHC-Frozen section of Rat Brain shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended concentration, 0.02-0.05µg/ml.