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Anti-Placental alkaline phosphatase antibody [C2]

Anti-Placental alkaline phosphatase antibody [C2]

Catalog Number:

A001477903STJ

Mfr. No.:

STJ16102633

Price:

$451

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Overview
- Please contact us at for specific academic pricing.
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG1
  
  Reactivity
  
  human
  
  Clone
  
  C2
  
  Immunogen
  
  A BALB/c mouse was immunized with PLAP, purified from FS phenotype human placenta.Fusion partner: X63-Ag8.P635.
  
  Purification
  
  The antibody is purified from tissue culture and contains no host Ig. Affinity purified from tissue culture.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS with 0.02% Sodium Azide.
  
  Storage
  
  Store for up to 1 year at 2-8°C upon receipt.
  
  Concentration
  
  Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
  
  Antigen
  
  (UniProt:P05187)
  
  * For Research Use Only (RUO). Not Used in Diagnostic or Therapeutic Applications.

Applications

Application

ELISA; FC; WB; IHC; IP

Application	Dilutions
ELISA	solid phase: not known. Tracer: 0.001-100 µg/ml for 30 min at RT
Flow cytometry	0.5-1.0 µg/million cells in 0.1 ml
Immunoblotting	1.0-2.0 µg/ml
Immunohistology	formalin-fixed: 2-4 µg/ml for 30 min at RT, staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Immunoprecipitation	1-2 µg per 100-500 µg of total cell lysate protein/1 ml of anti-mouse coated Sepharose-4B suspension

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