Name: Anti-PIAS2 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    E3 Sumo-Protein Ligase Pias2; Androgen Receptor-Interacting Protein 3; Arip3; Dab2-Interacting Protein; Dip; E3 Sumo-Protein Transferase Pias2; Msx-Interacting Zinc Finger Protein; Miz1; Pias-Ny Protein; Protein Inhibitor Of Activated Stat X; Protein Inhibitor Of Activated Stat2 (Gene ID:9063; UniProt:O75928)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-Preliminary experiments gave an approx 90kDa band in lysates of cell line A431after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.2kDa according to NP_004662.2. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
    IHC-In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5µg/ml.