Name: Anti-PI 3-kinase p8 Alpha/gamma antibody
Price: 224.00 USD

Overview
- PI 3-kinase p85 alpha/gamma is a protein encoded by the PIK3R1 gene which is approximately 83, 5 kDa. PI 3-kinase p85 alpha/gamma is localised to the cytoplasm, nucleus and cis-Golgi network. It is involved in RET signalling, the IL-2 pathway, downstream signalling of activated FGFR2 and regulation of lipid metabolism. It binds to activated protein-Tyr kinases, through its SH2 domain, and acts as an adapter by mediating the association of the p110 catalytic unit to the plasma membrane. It is also necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. PI 3-kinase p85 alpha/gamma isoform 2 is expressed in skeletal muscle and brain. Mutations in the PIK3R1 gene may result in Short syndrome and Pasli disease. STJ95078 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PI 3-kinase p85 alpha/gamma protein.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat; monkey; chicken
  
  Immunogen
  
  Synthesized peptide derived from human PI 3-kinase p85/p55 around the non-phosphorylation site of Y458/199.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha; Pi3-Kinase Regulatory Subunit Alpha; Pi3k Regulatory Subunit Alpha; Ptdins-3-Kinase Regulatory Subunit Alpha; Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha; Pi3-Kinase Subunit P85-Alpha; Ptdins-3-Kinase Regulatory Subunit P85-Alpha (Gene ID:5295; UniProt:P27986)
  
  * For research use only

Applications

Application

WB; IHC (paraffin); IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:20000

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Western blot analysis of lysates from COS7 cells, treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using PI3-kinase p85-alpha/gamma Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PI3-kinase p85-alpha/gamma Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of various cells using PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody diluted at 1：1000 Western blot analysis of 293T COLO lysis using PI 3-kinase p85 Alpha/Gamma antibody.

Anti-PI 3-kinase p8 Alpha/gamma antibody

Anti-PI 3-kinase p8 Alpha/gamma antibody