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Overview
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Stat3 is a protein encoded by the STAT3 gene which is approximately 88 kDa. Stat3 is localised to the cytoplasm and nucleus. It is involved in the immune response IL-23 signalling pathway, RET signalling, Th17 cell differentiation and prolactin signalling pathway. This protein falls under the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. Stat3 is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Mutations in the STAT3 gene may result in Hyperimmunoglobulin E recurrent infection syndrome. STJ90411 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Stat3 protein which only binds about Y705 when Y705 is phosphorylated.
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Overview
Western blot analysis of lysates from HeLa cells, using STAT3 (Phospho-Tyr705) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of KB cells using Phospho-Stat3 (Y705) Polyclonal Antibody diluted at 1：2000 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT3 (Phospho-Tyr705) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Stat3 (phospho Tyr705) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of various cells using Phospho-Stat3 (Y705) Polyclonal Antibody diluted at 1：2000 Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, Stat3 (phospho Tyr705) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Stat3 (phospho Tyr705) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of our customer's samples using primary antibody diluted at 1:500, The picture was kindly provided by our customer.. Cell lysate was extracted by Minute Plasma Membrane Protein Isolation and Cell Fractionation Kit (SM-005, Inventbiotech, MN, USA).
Immunofluorescence analysis of mouse-spleen tissue. 1, Stat3 (phospho Tyr705) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of mouse-spleen tissue. 1, Stat3 (phospho Tyr705) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Anti-Phospho-Stat3 (Y705) antibody
Anti-Phospho-Stat3 (Y705) antibody
Catalog Number:
A001045783STJ
Mfr. No.:
STJ90411
Price:
$224
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