Name: Anti-Phospho-PI 3-kinase p8 Alpha (Y607) antibody
Price: 224.00 USD

Overview
- PI 3-kinase p85 alpha is a protein encoded by the PIK3R1 gene which is approximately 83, 5 kDa. PI 3-kinase p85 alpha is localised to the cytoplasm, cytosol and nucleus. It is involved in RET signalling, the IL-2 pathway, downstream signalling of activated FGFR2, regulation of lipid metabolism and insulin signalling-generic cascades. It binds to activated protein-Tyr kinases, through its SH2 domain, and acts as an adapter in mediating the association of the p110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. PI 3-kinase p85 alpha isoform 2 and isoform 4 are present in skeletal muscle. Mutations in the PIK3R1 gene may result in Short syndrome and Agammaglobulinemia. STJ90925 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous PI 3-kinase p85 alpha protein which only binds about Y607 when Y607 is phosphorylated.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat; chicken
  
  Immunogen
  
  Synthesized peptide derived from human PI 3-kinase p85alpha around the phosphorylation site of Y607.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha; Pi3-Kinase Regulatory Subunit Alpha; Pi3k Regulatory Subunit Alpha; Ptdins-3-Kinase Regulatory Subunit Alpha; Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha; Pi3-Kinase Subunit P85-Alpha; Ptdins-3-Kinase Regulatory Subunit P85-Alpha (Gene ID:5295; UniProt:P27986)
  
  * For research use only

Applications

Application

IF; WB; IHC (paraffin); ELISA

Application	Dilutions
IF	1:50-200
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:10000

Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PI 3-kinase p8 Alpha (Y607) antibody

Anti-Phospho-PI 3-kinase p8 Alpha (Y607) antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PI 3-kinase p8 Alpha (Y607) antibody

Anti-Phospho-PI 3-kinase p8 Alpha (Y607) antibody

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