Name: Anti-Phospho-mTOR (S2448) antibody
Price: 224.00 USD

Overview
- mTOR is a protein encoded by the MTOR gene which is approximately 288, 9 kDa. mTOR is localised to the endoplasmic reticulum membrane and mitochondrion outer membrane. It is involved in RET signalling, regulation of lipid metabolism, mTOR signalling and glioma. This protein falls under the phosphatidylinositol kinase family. It is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. mTOR is expressed in numerous tissues, with highest levels expressed in the testis. Mutations in the MTOR gene may result in Smith-Kingsmore syndrome. STJ90336 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous mTOR protein which only binds about S2448 when S2448 is phosphorylated.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human mTOR around the phosphorylation site of S2448.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Serine/Threonine-Protein Kinase Mtor; Fk506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; Fkbp12-Rapamycin Complex-Associated Protein; Mammalian Target Of Rapamycin; Mtor; Mechanistic Target Of Rapamycin; Rapamycin And Fkbp12 Target 1; Rapamycin Target Protein 1 (Gene ID:2475; UniProt:P42345)
  
  * For research use only

Applications

Application

WB; IHC (paraffin); IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:40000

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Western blot analysis of various cells using Phospho-mTOR (S2448) Polyclonal Antibody diluted at 1：1000 Immunohistochemistry analysis of paraffin-embedded human brain, using mTOR (Phospho-Ser2448) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using mTOR (Phospho-Ser2448) Antibody. The lane on the right is blocked with the phospho peptide.

Immunofluorescence analysis of Hela cell. 1, mTOR (phospho Ser2448) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-mTOR (S2448) antibody

Anti-Phospho-mTOR (S2448) antibody