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Overview
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This antibody was cross-adsorbed to a phospho-tyrosine peptide before affinity purification using phospho-p38 Alpha (Tyr-323) peptide (without carrier). The antibody detects a 38 kDa* band corresponding to p38 Alpha on SDS-PAGE immunoblots of human Jurkat, K562, and mouse macrophage (J774A.1) cells treated with pervanadate. In addition, the antibody detects recombinant p38 MAP Kinase when treated with Fyn kinase (The in vitro kinase model was kindly provided by Dr. Paul Mittelstadt from the Laboratory of Immune Cell Biology at the NCI.)
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