Name: Anti-Phospho-Chk2 (T68) antibody
Price: 224.00 USD

Overview
- Chk2 is a protein encoded by the CHEK2 gene which is approximately 60, 9 kDa. Chk2 is localised to the nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, regulation of TP53 activity and apoptotic pathways. It is a serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Chk2 is highly expressed in the testis, spleen, colon and peripheral blood leukocytes. Mutations in the CHEK2 gene may result in Li-Fraumeni syndrome. STJ90225 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Chk2 protein only when phosphorylated at T68. This primary antibody specifically binds to endogenous Chk2 protein which only binds about T68 when T68 is phosphorylated.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human Chk2 around the phosphorylation site of T68.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Serine/Threonine-Protein Kinase Chk2; Chk2 Checkpoint Homolog; Cds1 Homolog; Hucds1; Hcds1; Checkpoint Kinase 2 (Gene ID:11200; UniProt:O96017)
  
  * For research use only

Applications

Application

IF; WB; IHC (paraffin); ELISA

Application	Dilutions
IF	1:50-200
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:20000

Western blot analysis of lysates from Jurkat cells treated with UV, using Chk2 (Phospho-Thr68) Antibody. The lane on the right is blocked with the phospho peptide.

Western blot analysis of various lysis using Phospho-Chk2 (T68) Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Chk2 (Phospho-Thr68) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of various cells using Phospho-Chk2 (T68) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Western blot analysis of HELA cells using Phospho-Chk2 (T68) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-Chk2 (T68) antibody

Anti-Phospho-Chk2 (T68) antibody

Western blot analysis of lysates from Jurkat cells treated with UV, using Chk2 (Phospho-Thr68) Antibody. The lane on the right is blocked with the phospho peptide.

Western blot analysis of various lysis using Phospho-Chk2 (T68) Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Chk2 (Phospho-Thr68) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of various cells using Phospho-Chk2 (T68) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Western blot analysis of HELA cells using Phospho-Chk2 (T68) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-Chk2 (T68) antibody

Anti-Phospho-Chk2 (T68) antibody

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