Name: Anti-Phospho-BRAF-Ser-446 antibody
Price: 596.00 USD

Overview
- This antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide. The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or A431 cells treated with Calyculin A. The antibody also detects other phosphorylated proteins at 150 and 200 kDa in calyculin A treated cells.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).
  
  Purification
  
  Antigen Affinity Purified
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
  
  Storage
  
  Store at -20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
  
  Antigen
  
  (UniProt:P15056)
  
  * For Research Use Only (RUO). Not Used in Diagnostic or Therapeutic Applications.

Applications

Application	Dilutions
WB	1:1000

Anti-Phospho-BRAF-Ser-446 antibody