Name: Anti-Phospho-AMP Alpha/2 (T183/172) antibody
Price: 224.00 USD

Overview
- AMPK alpha1/2 is a protein encoded by the PRKAA1 gene which is approximately 64 kDa. AMPK alpha1/2 is localised to the cytoplasm and nucleus. It is involved in RET signalling, mTOR signalling, AMP-activated protein kinase signalling and regulation of TP53 activity. It is an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, it activates energy-producing pathways and inhibits energy-consuming processes such as protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK alpha1/2 is expressed in the nervous system, liver, blood, intestine and kidney. Mutations in the PRKAA1 gene may result in obesity. STJ90735 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous AMPK alpha1/2 protein which only binds about T183/172 when T183/172 is phosphorylated.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat; monkey; pig
  
  Immunogen
  
  Synthesized peptide derived from human AMPKalpha1/2 around the phosphorylation site of T183/172.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1 (Gene ID:5562; UniProt:Q13131)
  
  * For research use only

Applications

Application

IF; WB; IHC (paraffin); ELISA

Application	Dilutions
IF	1:50-200
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:40000

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AMPK alpha (Phospho-Thr172) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-AMP Alpha/2 (T183/172) antibody

Anti-Phospho-AMP Alpha/2 (T183/172) antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AMPK alpha (Phospho-Thr172) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-AMP Alpha/2 (T183/172) antibody

Anti-Phospho-AMP Alpha/2 (T183/172) antibody

We Also Recommend

Anti-Phospho-GABAA Beta (S434) antibody

Anti-Phospho-MAPKAPK-2 (T334) antibody

Anti-Phospho-Shc (Y349) antibody