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Overview
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This antibody was cross-absorbed to unphospho-Ago2 (Ser-387) , before affinity purification using phospho-Ago2 (Ser-387) peptide (without carrier). The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of phosphorylated Ago2 on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A and mouse recombinant Ago2 full length protein. This reactivity is not observed after lambda phosphatase dephosphorylation.
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