Name: Anti-P4HA1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Prolyl 4-Hydroxylase Subunit Alpha-1; 4-Ph Alpha-1; Procollagen-Proline-2-Oxoglutarate-4-Dioxygenase Subunit Alpha-1 (Gene ID:5033; UniProt:P13674)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx 60kDa band observed in lysates of cell lines HEK293 and A431. Recommended concentration: 0.1-0.1µg/ml.An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.
    IHC-Paraffin embedded Human Skin and Spleen. Recommended concentration: 5µg/ml.