Name: Anti-NFIL3 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Nuclear Factor Interleukin-3-Regulated Protein; E4 Promoter-Binding Protein 4; Interleukin-3 Promoter Transcriptional Activator; Interleukin-3-Binding Protein 1; Transcriptional Activator Nf-Il3a (Gene ID:4783; UniProt:Q16649)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:2000.
    WB-Approx. 60kDa band observed in nuclear lysates of cell line Jurkat and HeLa. This molecular weight is routinely observed by other sources. An additional band of 30kDa was observed in lysates of cell line HeLa, which was completely blocked with the immunizing peptide. Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
    IF-
    FC-Flow cytometric analysis of Human peripheral blood mononuclear cells. Recommended concentration: 10ug/ml.