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STJ70314 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The STJ70314 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The

Anti-Neuro-d4/DPF1 antibody

Anti-Neuro-d4/DPF1 antibody

Catalog Number:

A001042124STJ

Mfr. No.:

STJ70314

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Zinc Finger Protein Neuro-D4; Brg1-Associated Factor 45b; Baf45b; D4-Zinc And Double Phd Fingers Family 1 (Gene ID:8193; UniProt:Q92782)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IF
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:2000.
    WB-Preliminary experiments showed a band at approx 38-40kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1µg/ml antibody staining (calculated MW of 37.9kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.
    IF-Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10µg/ml.

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