Name: Anti-NCF1/p47phox antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Neutrophil Cytosol Factor 1; Ncf-1; 47 Kda Autosomal Chronic Granulomatous Disease Protein; 47 Kda Neutrophil Oxidase Factor; Ncf-47k; Neutrophil Nadph Oxidase Factor 1; Nox Organizer 2; Nox-Organizing Protein 2; Sh3 And Px Domain-Containing Protein 1a; P47-Phox (Gene ID:653361; UniProt:P14598)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:20000.
    WB-Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates. This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate. Preliminary testing was unsuccessful on Rat Spleen and Thymus for this particular batch.
    IHC-Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5µg/ml.
    IF-Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.