Name: Anti-Munc13-4/UNC13D antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Protein Unc-13 Homolog D; Munc13-4 (Gene ID:201294; UniProt:Q70J99)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
    IHC-In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5µg/ml.In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data kindly provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA.Recommended concentration: 1-2µg/ml.

STJ70966 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

STJ70966 (2µg/ml) staining of Human T-lymphocyte lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

STJ70966 (0.5µg/ml) staining of paraffin embedded Mouse Lung (wt in A and KO in B) .

Anti-Munc13-4/UNC13D antibody

Anti-Munc13-4/UNC13D antibody

STJ70966 (2.5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

STJ70966 (2µg/ml) staining of Human T-lymphocyte lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

STJ70966 (0.5µg/ml) staining of paraffin embedded Mouse Lung (wt in A and KO in B) .

Anti-Munc13-4/UNC13D antibody

Anti-Munc13-4/UNC13D antibody

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