Name: Anti-Mouse PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12)
Price: 680.00 USD

- Overview
  - Antigen Distribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells
    Specificity: Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
    Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
- Properties
  - Host
    
    rat
    
    Isotype
    
    IgG2a kappa
    
    Reactivity
    
    mouse
    
    Clone
    
    29F.1A12
    
    Immunogen
    
    PD-1 cDNA followed by PD-1-Ig fusion protein
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
    
    Storage
    
    anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    1.0 - 5.0 mg/ml
    
    Regulatory Status
    
    Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml.
    Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear.
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:Programmed cell death protein 1, Pdcd1, CD279)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    Flow Cytometry; WB; Blocking; IHC (frozen)

Anti-Mouse PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12)

Anti-Mouse PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12)

Background

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