Name: Anti-Mouse IL-4 In Vivo Antibody - Low Endotoxin (11B11)
Price: 680.00 USD

- Overview
  - Specificity: Anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Rat IgG1 Isotype Control for In Vivo - Low Endotoxin [GL113] (ICH2246)
    Endotoxin: <0.5 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption. IL-4 is critical for inducing allergic responses. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.
- Properties
  - Host
    
    rat
    
    Isotype
    
    IgG1 kappa
    
    Reactivity
    
    mouse
    
    Clone
    
    11B11
    
    Immunogen
    
    Purified Recombinant Mouse IL-4 (>98%)
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
    
    Storage
    
    anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) is stable when stored at 2-8°C for 1 week. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    1.0 - 5.0 mg/ml
    
    Regulatory Status
    
    ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml.
    ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 µg/ml is recommended.
    Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo.
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:Interleukin-4, Il-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    ELISA Capture; ELISPOT; Flow Cytometry; Neutralization; Immunoprecipitation; Functional Assays; CyTOF; IHC

Anti-Mouse IL-4 In Vivo Antibody - Low Endotoxin (11B11)

Anti-Mouse IL-4 In Vivo Antibody - Low Endotoxin (11B11)

Background

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