Anti-Mouse GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9)

Anti-Mouse GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9)

Catalog Number:
A001437277ICH
Mfr. No.:
ICH1125
Price:
$680
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      • Overview
        • Specificity: Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) recognizes an epitope on Mouse GM-CSF
          Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype
          Isotype Control: Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
          Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
          Aggregation: Aggregation level ≤ 5%

          Please contact us at for specific academic pricing.

          Background

          Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.

          GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.

      • Properties
        • Host
          rat
          Isotype
          IgG2a
          Reactivity
          mouse
          Clone
          MP1-22E9
          Immunogen
          Yeast Derived Recombinant Mouse GM-CSF
          Purification
          >95% by SDS-PAGE and HPLC
          Formulation
          0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
          Storage
          anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) is lyophilized and can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
          Molecular Weight
          1.0 - 5.0 mg/ml
          Regulatory Status
          Western Blotting: To detect Mouse GM-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml.Each investigator should determine their own optimal working dilution for specific applications.

          * Products are for research use only. Not for use in diagnostic or therapeutic procedures.

      • Applications
        • Application
          WB; ELISA; ELISPOT; Blocking; Immunocytochemistry; IHC (frozen)

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