Anti-Mouse, Human CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2)

Anti-Mouse, Human CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2)

Catalog Number:
A001437279ICH
Mfr. No.:
ICH1130
Price:
$680
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      • Overview
        • Antigen Distribution: The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .
          Specificity: Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.
          Isotype Control: Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
          Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
          Aggregation: Aggregation level ≤ 5%

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          Background

          CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.

      • Properties
        • Host
          rat
          Isotype
          IgG2a
          Reactivity
          mouse; human
          Clone
          RA3-6B2
          Immunogen
          Abelson murine leukemia virus-induced pre-B tumor cells
          Purification
          >95% by SDS-PAGE and HPLC
          Formulation
          No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
          Storage
          Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) is stable for at least four (4) weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
          Molecular Weight
          0.5 mg/ml
          Regulatory Status
          Functional Assay: In vivo treatment with Rat Anti-Mouse CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity.
          Each investigator should determine their own optimal working dilution for specific applications.

          * Products are for research use only. Not for use in diagnostic or therapeutic procedures.

      • Applications
        • Application
          WB; Functional Assays; Flow Cytometry; IHC (frozen); IHC (paraffin); Immunoprecipitation; Immunofluorescence; ELISA

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