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Overview
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MDM2 is a protein encoded by the MDM2 gene which is approximately 55, 2 kDa. MDM2 is localised to the nucleus and cytoplasm. It is involved in RET signalling, GAB1 signalosome and the regulation of TP53 activity. It is a nuclear-localized E3 ubiquitin ligase that can promote tumour formation by targeting tumour suppressor proteins, such as p53, for proteasomal degradation. It is also a component of the TRIM28/KAP1-ERBB4-MDM2 complex which links the growth factor and DNA damage response pathways. MDM2 is ubiquitously expressed in human tissues. Mutations in the MDM2 gene may result in accelerated tumour formation. STJ96551 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of MDM2 protein.
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Overview
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100 Western blot analysis of lysate from mouse liver cells, using MDM2 Antibody.
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100 Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100 Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, MDM2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of mouse liver cells using MDM2 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000 Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, MDM2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1, MDM2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, MDM2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, MDM2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, MDM2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of human-kidney-cancer tissue. 1, MDM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-liver-cancer tissue. 1, MDM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Anti-MDM2 antibody
Anti-MDM2 antibody
Catalog Number:
A001051921STJ
Mfr. No.:
STJ96551
Price:
$224
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