![Western blot analysis of extracts from normal (control) and [KO Validated] IRF3 Rabbit pAb knockout (KO) HeLa cells, using [KO Validated] IRF3 Rabbit pAb antibody (STJ112965) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](https://resource.amerigoscientific.com/p-img/STJ112965_2_WB__44332.1602778197.jpg)
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Overview
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This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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