Name: Anti-ICOS antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Inducible T-Cell Costimulator; Activation-Inducible Lymphocyte Immunomediatory Molecule; Cd Antigen Cd278 (Gene ID:29851; UniProt:Q9Y6W8)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Preliminary experiments gave an approx 13kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.6kDa according to NP_036224.1. The 13kDa band was successfully blocked by incubation with the immunizing peptide.
    IHC-In paraffin embedded Human Lymph Node shows membrane staining just outside the mantle zone of the Germinal Centre. Recommended concentration: 1-2µg/ml. Paraffin embedded Human Tonsil and Thymus. Recommended concentration: 3.75µg/ml.

STJ71963 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-ICOS antibody

Anti-ICOS antibody

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