Name: Anti-GATA1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Erythroid Transcription Factor; Eryf1; Gata-Binding Factor 1; Gata-1; Gf-1; Nf-E1 Dna-Binding Protein (Gene ID:2623; UniProt:P15976)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Approx 50-55kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8kDa according to NP_002040.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Hippocampus lysate.
    IF-Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

STJ70751 (1µg/ml) staining of K562 nuclear cell lysate (A) and negative control Human Hippocampus (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-GATA1 antibody

Anti-GATA1 antibody

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