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Overview
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eIF4A1 is a protein encoded by the EIF4A1 gene which is approximately 46, 1 kDa. eIF4A1 is localised to the cytoplasm and cell membrane. It is involved in viral mRNA translation, deadenylation-dependent mRNA decay, regulation of lipid metabolism and insulin signalling-generic cascades. It is an ATP-dependent RNA helicase that is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. eIF4A1 is expressed in the liver, lung, nervous system, pancreas and skin. STJ97038 was developed from clone M8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous eIF4A1 protein.
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Overview
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Western blot analysis of 1) 293T, 2) Hela, 3) HepG2, 4) Mouse Brain tissue Immunofluorescence analysis of Mouse-spleen tissue. 1, eIF4A1 monoclonal antibody (M8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Anti-eIF4A1 antibody
Anti-eIF4A1 antibody
Catalog Number:
A001052407STJ
Mfr. No.:
STJ97038
Price:
$224
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