Name: Anti-EGFR antibody
Price: 224.00 USD

Overview
- EGFR is a protein encoded by the EGFR gene which is approximately 134, 3 kDa. EGFR is localised to the cell membrane. and endoplasmic reticulum membrane. It is involved in RET signalling, apoptotic pathways in synovial fibroblasts, glioma and the GPCR pathway. It is a transmembrane glycoprotein that is a member of the protein kinase superfamily. It binds to epidermal growth factor and this induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. EGFR is expressed ubiquitously with isoform 2 also being expressed in ovarian cancers. Mutations in the EGFR gene may result in lung cancer and Cowden syndrome. STJ92862 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of EGFR.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human EGFR around the non-phosphorylation site of Y1016.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 (Gene ID:1956; UniProt:P00533)
  
  * For research use only

Applications

Application

WB; IHC; IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:5000

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EGFR Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using EGFR Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HUVEC cells, treated with Serum 20% 30', using EGFR Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of human-liver tissue. 1, EGFR Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EGFR antibody

Anti-EGFR antibody