Name: Anti-E Beta antibody
Price: 224.00 USD

Overview
- ER beta is a protein encoded by the ESR2 gene which is approximately 59, 2 kDa. ER beta is localised to the nucleus. It is involved in gene expression, the nuclear receptor transcription pathway, development endothelin-1/EDNRA signalling and the prolactin signalling pathway. This protein falls under the family of oestrogen receptors. It binds oestrogens and activates expression of reporter genes containing oestrogen response elements in an oestrogen-dependent manner. Oestrogen controls many cellular processes including growth, differentiation and function of the reproductive system. Oestrogen is also responsible for the growth and maintenance of the skeleton and the normal function of the cardiovascular and nervous systems. ER beta isoform 1 is expressed in testis and ovaries while isoform 2 is expressed in spleen, thymus, testis and ovaries. Mutations in the ESR2 gene may result in medullary thyroid carcinoma and de quervain disease. STJ93002 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of ER beta protein.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human ERbeta around the non-phosphorylation site of S105.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2 (Gene ID:2100; UniProt:Q92731)
  
  * For research use only

Applications

Application

WB; IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IF	1:200-1:1000
ELISA	1:10000

Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using Estrogen Receptor-beta Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunofluorescence analysis of HeLa cells, using Estrogen Receptor-beta Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of mouse-kidney tissue. 1, ER Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-E Beta antibody

Anti-E Beta antibody

Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using Estrogen Receptor-beta Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunofluorescence analysis of HeLa cells, using Estrogen Receptor-beta Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of mouse-kidney tissue. 1, ER Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-E Beta antibody

Anti-E Beta antibody

We Also Recommend

Anti-ETAR antibody

Anti-Ethanolamine kinase 2 antibody

Anti-ETL antibody