Name: Anti-Dynactin antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Dynactin Subunit 1; 150 Kda Dynein-Associated Polypeptide; Dap-150; Dp-150; P135; P150-Glued (Gene ID:1639; UniProt:Q14203)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; Flow Cytometry; IF; ICC; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx 150kDa band observed in Human Cerebellum lysates. Additional bands between approx. 40-70kDa were consistently observed, and all were successfully blocked by incubation with the immunizing peptide. Recommended concentration 1-2µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.
    IHC-In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4µg/ml. A customer found positive results in IHC on Human HeLa cells.
    IF-Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts.
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.