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STJ71102 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai STJ71102 (2µg/ml) staining of PFA-fixed cryo-sectioned Mouse Hippocampus. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.

STJ71102 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Anti-DIO2 antibody

Anti-DIO2 antibody

Catalog Number:

A001042895STJ

Mfr. No.:

STJ71102

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; pig
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Type II Iodothyronine Deiodinase; 5dII; DioII; Type 2 Di; Type-II 5'-Deiodinase (Gene ID:1734; UniProt:Q92813)
    
    * For research use only
- Applications
  - Application
    
    ELISA; Flow Cytometry; IF; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    IHC-Cryo-sectioned Mouse Brain shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3µg/ml. Data obtained from customer.
    IF-Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

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