Name: Anti-Connexin 43 antibody
Price: 224.00 USD

Overview
- Connexin 43 is a protein encoded by the GJA1 gene which is approximately 43 kDa. Connexin 43 is localised to the cell membrane and endoplasmic reticulum. It is involved in gap junction trafficking and transport of connexins along the secretory pathway. It is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. In the heart these gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is expressed in the heart and foetal cochlea. Mutations in the GJA1 gene may result in Oculodentodigital dysplasia. STJ92411 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Connexin 43 protein.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human Connexin 43
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Gap Junction Alpha-1 Protein; Connexin-43; Cx43; Gap Junction 43 Kda Heart Protein (Gene ID:2697; UniProt:P17302)
  
  * For research use only

Applications

Application

WB; IHC (paraffin); IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:10000

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Connexin 43 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunofluorescence analysis of A549 cells, using Connexin 43 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using Connexin 43 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1, Connexin 43 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Connexin 43 antibody

Anti-Connexin 43 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Connexin 43 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunofluorescence analysis of A549 cells, using Connexin 43 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using Connexin 43 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1, Connexin 43 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Connexin 43 antibody

Anti-Connexin 43 antibody

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