Name: Anti-Clusterin/APOJ antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Clusterin; Aging-Associated Gene 4 Protein; Apolipoprotein J; Apo-J; Complement Cytolysis Inhibitor; Cli; Complement-Associated Protein Sp-40-40; Ku70-Binding Protein 1; Na1/Na2; Sulfated Glycoprotein 2; Sgp-2; Testosterone-Repressed Prostate Message 2; Trpm-2 Cleaved Into-Clusterin Beta Chain; Apojalpha; Complement Cytolysis Inhibitor A Chain-Clusterin Alpha Chain; Apojbeta; Complement Cytolysis Inhibitor B Chain (Gene ID:1191; UniProt:P10909)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:64000.
    WB-Approx 35-37kDa band observed in Human Colon and Prostate lysates. The observed molecular weight corresponds to earlier findings in literature. Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
    IF-Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml

STJ70776 (0.1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

STJ70776 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing ER/cytoplasmic staining. The nuclear stain is DAPI

Anti-Clusterin/APOJ antibody

Anti-Clusterin/APOJ antibody

STJ70776 (0.1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

STJ70776 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing ER/cytoplasmic staining. The nuclear stain is DAPI

Anti-Clusterin/APOJ antibody

Anti-Clusterin/APOJ antibody

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