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Immunofluorescence analysis of 293T cells using Cyclin B1 antibody (STJ118478) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Cyclin B1 antibody (STJ118478). Western blot was performed from the immunoprecipitate using Cyclin B1 antibody (STJ118478) at a dilition of 1:1000.

Immunohistochemistry of paraffin-embedded mouse testis using Cyclin B1 antibody (STJ118478) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using Cyclin B1 antibody (STJ118478) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin B1 antibody (STJ118478) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using Cyclin B1 antibody (STJ118478) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (STJ118478) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-CCNB1 antibody

Anti-CCNB1 antibody

Catalog Number:

A001025252STJ

Mfr. No.:

STJ118478

Price:

$297

Size:

Quantity:

Add to Cart:

Overview
- Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172. 1).
  
  Purification
  
  Affinity purification
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  
  Storage
  
  Store at -20°C and avoid freeze-thaw cycles.
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  G2/Mitotic-Specific Cyclin-B1 (Gene ID:891; UniProt:P14635)
  
  * For research use only

Applications

Application

WB; IHC; IF; IP

Application	Dilutions
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
IP	1:50-1:100

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