Name: Anti-Calnexin antibody
Price: 224.00 USD

Overview
- Calnexin is a protein encoded by the CANX gene which is approximately 67, 5 kDa. Calnexin is localised to the endoplasmic reticulum membrane. It is involved in class I MHC mediated antigen processing and presentation, assembly of viral components at the budding site and the calnexin/calreticulin cycle. It is a calcium-binding, endoplasmic reticulum-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It also plays a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Calnexin is expressed in the nervous system, liver, skin, blood and lung. Mutations in the CANX gene may result in Frey syndrome and orbital melanoma. STJ91978 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Calnexin protein.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  Synthesized peptide derived from human Calnexin around the non-phosphorylation site of S583.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Calnexin; Ip90; Major Histocompatibility Complex Class I Antigen-Binding Protein P88; P90 (Gene ID:821; UniProt:P27824)
  
  * For research use only

Applications

Application

WB; IHC (paraffin); IF; ELISA

Application	Dilutions
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:5000

Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using Calnexin Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Calnexin antibody

Anti-Calnexin antibody

Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using Calnexin Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Calnexin Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Calnexin antibody

Anti-Calnexin antibody

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