Name: Anti-Calnexin antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Calnexin; Ip90; Major Histocompatibility Complex Class I Antigen-Binding Protein P88; P90 (Gene ID:821; UniProt:P27824)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IF; EIA
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx. 100kDa band observed in Mouse Heart, Human Brain, NIH3T3 and HeLa lysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies :13691-6., PMID: 8662691). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Heart and Brain, and Pig for this particular batch.
    IHC-In paraffin embedded Human Kidney shows textured staining in the cytoplasm of DCT cells. Recommended concentration: 3-6µg/ml. Paraffin embedded Human Testis and Uterus. Recommended concentration: 3.75µg/ml.
    IF-Strong expression of the protein seen in the cytoplasm and endoplasmic reticulum of NIH3T3 cells. Recommended concentration: 5µg/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Calnexin captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml