Name: Anti-betaCstF-64 variant 1/betaCstF-64 variant 3 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    mouse
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    * For research use only
- Applications
  - Application
    
    ELISA; IP
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Immunoprecipitation: In transfected HeLa transiently expressing Mouse ßCstf-64, a band of approx. 80kDa was observed using anti-DYKDDDDK antibody in Western blot after immunoprecipitation with EB11838. No bands were observed in mock-transfected HeLa. Yields were similar between RIPA buffer and Triton X-100 buffer conditions. Recommended amount, 2ug per lysate.Immunoprecipitation: An approx 75kDa band is observed when endogenously expressed ßCstf-64 is pulled down from Mouse Brain lysates using EB11838, while Cstf-64 which is endogenously expresed in Mouse Liver, is not pulled down. Western blot was probed with a mouse monoclonal antibody recognizing both ßCstf-64 anf Cstf-64 Recommended amount 2ug EB11838/500ug lysate protein.