Name: Anti-ATP1A1 antibody
Price: 297.00 USD

Overview
- The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+-ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A1 (NP_000692. 2).
  
  Purification
  
  Affinity purification
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  
  Storage
  
  Store at -20°C and avoid freeze-thaw cycles.
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Sodium/Potassium-Transporting Atpase Subunit Alpha-1; Na(+/K(+ Atpase Alpha-1 Subunit; Sodium Pump Subunit Alpha-1 (Gene ID:476; UniProt:P05023)
  
  * For research use only

Applications

Application

WB; IHC

Application	Dilutions
WB	1:500-1:1000
IHC	1:50-1:200

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Immunoprecipitation analysis of 200ug extracts of LO2 cells, using 3 ug ATP1A1 antibody (STJ22716). Western blot was performed from the immunoprecipitate using ATP1A1 antibody (STJ22716) at a dilition of 1:1000.

Immunohistochemistry of paraffin-embedded human stomach using ATP1A1 antibody (STJ22716) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of HeLa cells, using ATP1A antibody (STJ22716) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human prostate using ATP1A1 antibody (STJ22716) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ATP1A1 Antibody (STJ22716) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

Anti-ATP1A1 antibody

Anti-ATP1A1 antibody